restriction enzymes spei hf (New England Biolabs)
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restriction enzymes spei hf
Restriction Enzymes Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+enzymes+spei+hf/SpeI-HF/pm41498345-64-0-13
Average 96 stars, based on 1132 article reviews
Restriction Enzymes Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+enzymes+spei+hf/SpeI-HF/pm41498345-64-0-13
Average 96 stars, based on 1132 article reviews
restriction enzymes spei hf - by Bioz Stars,
2026-10
96/100 stars
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other:Article Title: Expression, Purification, and Microscopy-Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin. Article Snippet: Plasmid Preparation:Article Title: Myosin 10 uses its MyTH4 and FERM domains differentially to support two aspects of spindle pole biology required for mitotic spindle bipolarity Article Snippet: In Fusion HD cloning (Takara 638910) was used to switch full length WT mouse Myo10 in EGFP-C1 to mScarlet-C1, and to clone WT and mutant versions of mScarlet-Myo10 into an Xlone plasmid (Addgene plasmid # 96930). .. The Xlone-GFP backbone plasmid was linearized by treating with Article Title: Myosin 10 uses its MyTH4 and FERM domains differentially to support two aspects of spindle pole biology required for mitotic spindle bipolarity Article Snippet: In Fusion HD cloning (Takara 638910) was used to switch full length WT mouse Myo10 in EGFP-C1 to mScarlet-C1, and to clone WT and mutant versions of mScarlet-Myo10 into an Xlone plasmid (Addgene plasmid # 96930). .. The Xlone-GFP backbone plasmid was linearized by treating with Article Title: Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarity Article Snippet: In Fusion HD cloning (Takara 638910) was used to move full length WT mouse Myo10 in EGFP-C1 to mScarlet-C1, and to clone WT and mutant versions of mScarlet-Myo10 into an Xlone plasmid (Addgene plasmid # 96930). .. The Xlone-GFP backbone plasmid was linearized by treating with Article Title: Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarity Article Snippet: Myosin 10 (Myo10) couples microtubules and integrin-based adhesions to movement along actin filaments via its microtubule-binding MyTH4 domain and integrin-binding FERM domain, respectively.. Here we show that Myo10 depleted HeLa cells and mouse embryo fibroblasts (MEFs) both exhibit a pronounced increase in the frequency of multipolar spindles.. Staining of unsynchronized metaphase cells showed that the primary driver of spindle multipolarity in Myo10 depleted MEFs and in Myo10 depleted HeLa cells lacking supernumerary centrosomes is pericentriolar material (PCM) fragmentation, which creates y-tubulin-positive acentriolar foci that serve as extra spindle poles. |